Coding
Str(e)pE

Part:BBa_K4590006:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


UvrA-like protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1148
    Illegal NgoMIV site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 967
    Illegal BsaI.rc site found at 1909
    Illegal SapI site found at 357


Design Notes

The Urva-like protein chosen for overexpression is part of the cosmomycin cluster.


Source

Streptomyces olindensis strain DAUFPE 5622 2, whole genome shotgun sequence GenBank: JJOH01000002.1

References